We studied transcription by RNAP at physiological nucleotide concentrations using a new single-molecule assay together with improved optical trapping instrumentation. In combination, these achieve ...

Backtracking refers to the retrograde movement of the RNA-dependent RNA polymerase (RdRp), which is the crucial enzyme within the SARS-CoV-2 replication-transcription complex. This enzyme comprises ...

The research team found that while specific amino acid residues of RNA polymerase II are critical for backtracking, cleavage of the mis-incorporated nucleotide only requires the RNA itself (i.e.